Technical Data
Thrombospondin (TSP)
Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Suitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25 to 1:50 for 60 min at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.

MW of Antigen: ~450kD (non-reduced); 170 to 180kD (reduced)

Collagen Type V-binding domain of TSP

Positive Control:
Platelets in placenta, megakaryocytes in bone marrow.

Cellular Localization:
Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG13F353Affinity Purified
500ul4C (-20C Glycerol)Blue IceHumanMouse
Reduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets
Purified by Protein G affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06DX
Recognizes human TSP. Inhibits TSP-collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low conc. of Ca2+. It shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Species Crossreactivity: Bovine, porcine, equine, canine, sheep, mouse, and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit VM, et al. J Bio Chem, 1986, 261(4):19628. 2. Galvin NJ, et al. J Cell Bio, 1987, 104(5):141322. 3. Dixit VM, et al. Biochemistry, 1985, 24(16):42705