Technical Data
T5100-06D
Thrombospondin (TSP)
Description:
Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Applications:
Suitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25 to 1:50 for 60 min at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.

MW of Antigen: ~450kD (non-reduced); 170 to 180kD (reduced)

Epitope:
Collagen Type V-binding domain of TSP

Positive Control:
Platelets in placenta, megakaryocytes in bone marrow.

Cellular Localization:
Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13F353Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Reduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06DX
Specificity:
Recognizes human TSP. Inhibits TSP-collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low conc. of Ca2+. It shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Species Crossreactivity: Bovine, porcine, equine, canine, sheep, mouse, and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit VM, et al. J Bio Chem, 1986, 261(4):19628. 2. Galvin NJ, et al. J Cell Bio, 1987, 104(5):141322. 3. Dixit VM, et al. Biochemistry, 1985, 24(16):42705