Technical Data
Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)
Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25-1:50. Using an ABC Method. Incubation time is 30 minutes at RT.
Immunohistochemistry (Paraffin): 1:25-1:50. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil or placenta

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceHumanMouse
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum
Supplied as a liquid, sodium azide.
Recognizes the non-reduced form of human Thrombospondin at~450kD and the reduced form at ~170-180kD. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit et al. Biochemistry 24 (16): 4270, 1985. 2. Dixit et al. J Bio Chem 261 (4): 1962, 1986. 3. Galvin et al. J Cell Bio 104 (5): 1413, 1987