Technical Data
T5100-06H
Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)
Description:
Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25-1:50. Using an ABC Method. Incubation time is 30 minutes at RT.
Immunohistochemistry (Paraffin): 1:25-1:50. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil or placenta

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19F208Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum
Purity:
Purified
Form
Supplied as a liquid, sodium azide.
Specificity:
Recognizes the non-reduced form of human Thrombospondin at~450kD and the reduced form at ~170-180kD. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixit et al. Biochemistry 24 (16): 4270, 1985. 2. Dixit et al. J Bio Chem 261 (4): 1962, 1986. 3. Galvin et al. J Cell Bio 104 (5): 1413, 1987