Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Positive Control: HUVEC cells.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets. Epitope: N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.|
|10mM PBS [pH7.4] containing 0.09% sodium azide with 0.2%BSA|
|It shows no crossreaction with fibronectin, fibrinogen or Von Willebrand factor. Species Crossreactivity: Crossreacts with Human, Rat, Mouse, bovine and Chicken. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. DeFreitas MF; Yoshida CK; Frazier WA; Mendrick DL; Kypta RM; Reichardt LF. Identification of integrin alpha 3 beta 1 as a neuronal thrombospondin receptor mediating neurite outgrowth. Neuron, 1995 Aug, 15(2):333-43.|
2. RayChaudhury A; Frazier WA; D'Amore PA. Comparison of normal and tumorigenic endothelial cells: differences in thrombospondin production and responses to transforming growth factor-beta. Journal of Cell Science, 1994 Jan, 107 ( Pt 1):39-46.
3. Tolsma SS; Volpert OV; Good DJ; Frazier WA; Polverini PJ; Bouck N. Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity.
Journal of Cell Biology, 1993 Jul, 122(2):497-511.
4. Frazier WA. Thrombospondins. Current Opinion in Cell Biology, 1991 Oct, 3(5):792-9.
5. Prater CA; Plotkin J; Jaye D; Frazier WA. The properdin-like type I repeats of human thrombospondin contain a cell attachment site. Journal of Cell Biology, 1991 Mar, 112(5):1031-40.
6. Good DJ; Polverini PJ; Rastinejad F; Le Beau MM; Lemons RS; Frazier WA; Bouck NP. A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proceedings of the National Academy of Sciences of the United States of America, 1990 Sep, 87(17):6624-8.
7. Frazier WA. Thrombospondin: a modular adhesive glycoprotein of platelets and nucleated cells. Journal of Cell Biology, 1987 Aug, 105(2):625-32.
8. Santoro SA; Frazier WA. Isolation and characterization of thrombospondin. Methods in Enzymology, 1987, 144:438-46.
9.Prater CA, et. al. J Cell Biol, 1991, 112(5):1031-40.