Technical Data
T5100-06T
Thrombospondin (TSP)
Description:
Thrombospondin is a protein from platelet agranules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. This antibody inhibits TSP collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate, and heparin). It shows no cross reaction with fibronectin, fibrinogen, and von Willebrand factor. It shows a mild cross reactivity towards TSP 2.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil, placenta

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2D7Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human thrombospondin. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.