Technical Data
T5100-08A
Thrombospondin (TSP)
Description:
Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Applications:
Suitable for use in ELISA, Double Radial Immunodiffusion and Immunoelectrophoresis. Other applications not tested.

Recommended Dilution:
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted in 2mm diameter peripheral wells).
Immunoelectrophoresis: 120ul against 2ul serum or equivalent
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Thrombospondin, a glycoprotein, has been purified from platelet lysate.
Purity:
Serum
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 1ml sterile dH2O.
Specificity:
Recognizes human Thrombospondin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.