Technical Data
T5150-07
Thymic Stromal Lymphopoietin Protein Receptor (Cytokine Receptor-Like 2, TSLP-R)
Description:
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; cotransfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1. Despite its predicted molecular weight, TSLP-R often migrates at a higher molecular weight in SDS-PAGE.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml
Immunofluorescence: 20ug/ml
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to19aa near the center of human Thymic Stromal Lymphopoietin Protein Receptor (GenBank accession: Q9HC73).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human Thymic Stromal Lymphopoietin Protein Receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ziegler, S.F. and Liu, Y.-J. (2006) Nature Immunol. 7:709-714. 2. Sims, J.E., et al. (2000) J. Exp. Med. 192:671-680. 3. Levin, S.D., et al. (1999) J. Immunol. 162:677-683. 4. Park, L.S., et al. (2000) J. Exp. Med. 192:659-670. 5. Isaksen, D.E., et al. J. Immunol. 163:5971-5977.