Technical Data
T5151-09
Thymine Dimer (Cyclobutane Pyrimidine Dimer, CPD)
Description:
This antioody is intended for the detection of DNA damage as a result of UV irradiation.

Hybridoma:
Mouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) spleen cells

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Southern Blot, Northern Blot, and Dot Blot. Other applications have not been tested.

Recommended Dilution:
In situ Hybridization: 1:40-1:80. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended.
Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1KTM53Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
UV-irradiated salmon sperm DNA
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS containing 0.1% sodium azide and protein stabilizer
Specificity:
Recognizes thymine dimers produced by UV irradiation in double- or single- stranded DNA. Does not react with (6-4) photo products.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.