		Technical Data
T5151-09	Thymine Dimer (Cyclobutane Pyrimidine Dimer, CPD)

Description:
This antioody is intended for the detection of DNA damage as a result of UV irradiation.

Hybridoma:
Mouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) spleen cells

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Southern Blot, Northern Blot, and Dot Blot. Other applications have not been tested.

Recommended Dilution:
In situ Hybridization: 1:40-1:80. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended.
Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	KTM53	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.5mg/ml

Immunogen:

UV-irradiated salmon sperm DNA

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid in PBS containing 0.1% sodium azide and protein stabilizer

Specificity:

Recognizes thymine dimers produced by UV irradiation in double- or single- stranded DNA. Does not react with (6-4) photo products.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.