Technical Data
T5349
Thyroid Hormone Receptor, beta 1
|
Technical Data |
|
T5349 |
Thyroid Hormone Receptor, beta 1 |
|
|
Description: Thyroid hormone receptors (TRs) belong to a large superfamily of DNA-binding proteins, which includes the steroid hormone, vitamin D, and retinoic acid receptors. Members of this family share a similar domain organization that features a central DNA-binding domain containing two zinc fingers and carboxy-terminal ligand-binding domain. Three TR isoforms have been identified in the rat. THR, beta-1 and THR, beta-2 are encoded on the same gene and differ only at the amino-terminal region due to alternative splicing and promoter selection. THR, alpha-1, which is encoded on a separate gene, is homologous to the THR, beta in both the DNA and ligand-binding domains. Interestingly, an alternative splice product of THR, alpha-1, c-erbA, alpha-2, has a different carboxy-terminal region and does not bind thyroid hormone. Applications: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. Recommened Dilutions: Immunoprecipitation: Immunoprecipitates rat THR, beta-1 that has been translated in vitro. Immunohistochemistry: Positively stains nuclei of rat and human pituitary. Western Blot: 0.5-2ug/ml detects THR, beta-1 (p66) in 20ug of a HeLa nuclear extract. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibody: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||