Technical Data
T5400-49
Thyroid Stimulating Hormone (TSH)
Description:
Thyroid-stimulating hormone (TSH or thyrotropin) is a pituitary hormone of 28kD, which stimulates thyroid growth and production of thyroid hormones. An important indicator of thyroid function, TSH is used to monitor thyroid associated diseases. Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. This clone may be useful in the classification of pituitary adenomas and the differential identification of primary and metastatic tumors of the pituitary.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin, paraffin): 1:200-1:400 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Anterior pituitary

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F366Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified human TSH-b. Cellular Localization: Cytoplasmic. MW: 14kD (beta) 28kD (alpha/beta).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5400-49X.
Specificity:
Recognizes human Thyroid Stimulating Hormone (TSH). Labels thyrotropic cells of the pituitary.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.