Technical Data
TIA1 (Cytotoxic Granule-associated RNA binding protein, Nucleolysin TIA-1 isoform p40, p40-TIA-1, T-cell-restricted intracellular antigen-1, RNA-binding protein)
TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15kD cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkins, etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemias, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The authors concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkins and lymphoblastic leukemias were negative for TIA-1 (1).

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 1:50-1:100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval; an optional pepsin treatment can also improve the signal.
Optimal dilutions to be determined by the researcher.

Positive Control:
Anaplastic large cell lymphoma or tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceHumanMouse
Not Determined
Recombinant full length human TIA 1. Cellular localization: Cytoplasmic (granular).
Supplied as a liquid in proprietary buffer with protein carrier and sodium azide.
Recognizes human TIA 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.