Technical Data
TICAM-1 (Toll-interleukin 1 Receptor Domain (TIR)-containing Adaptor Molecule-1, TRIF, Toll/IL-1 Receptor Domain-containing Adapter-inducing IFN-beta)
TRIF is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses (4). TRIF, along with other molecules such as TIRP, TIRAP, and MyD88, serves as an adaptor protein to several of the TLR molecules. Following activation of TLR3 and TLR4, TRIF engages the kinase TBK1 and allows its subsequent activation of the interferon regulatory factor (IRF)-3 (5). TRIF is also involved in the activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-kB (6).

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutons:
Western Blot: 2-4ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to 14aa near the C-terminus of human TRIF. GenBank accession #NP_891549.
Purified by Ion Exchange chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human TRIF (TICAM-1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. O'Neill LAJ, Trends in Imm. 2003; 24:286-9. 2. Vogel SN,. Mol. Interv. 2003; 3:466-77. 3. Takeda K, et al Annu. Rev. Immunol. 2003; 21:335-76. 4. Janeway CA Jr and Medzhitov R. . Annu. Rev. Immunol. 2002; 20:197-216. 5. Fitzgerald KA, Nat. Immunol. 2003; 4:591-6. 6. Sato S, et al . J. Immunol. 2003; 171(8):4304-10.