Technical Data
TIF1 alpha (Transcriptional Intermediary Factor 1 alpha, TF1A, TIF1alpha, hTIF1, PTC6, RING Finger Protein 82, RNF82, Tripartite Motif-containing 24, TRIM24)
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains-a RING, a B-box type 1 and a B-box type 2-and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to amino acid residues 1,000 and the C-terminus(residue 1050) of human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24) using the numbering given in entry NP_056989.2 (GeneID 8805).
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Species Crossreactivity: Human. In initial testing, BL2795 failed to detect TIF1 Alpha/TRIM24 in lysate from mouse NIH3T3 cells. Other spcies have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.