Technical Data
T5498-93
TIF1 alpha (Transcriptional Intermediary Factor 1 alpha, TF1A, TIF1alpha, hTIF1, PTC6, RING Finger Protein 82, RNF82, Tripartite Motif-containing 24, TRIM24) (Azide Free)
Description:
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1,000
Immunohistochemistry (paraffin): 1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG311C880Affinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to a portion of amino acids within aa432-569 of human TRIM24.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human TRIM24.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.