		Technical Data
T5500	TIRAP (MAL, Toll-interleukin 1 Receptor Domain containing Adaptor Protein, TIRAP)

Description:
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production (4). It has 235 amino acids and is commonly seen as a 32-37 kD protein (4).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100
observed size: 32 kD
positive control: HUVEC cell lysate
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM	4H263	Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to a portion of human TIRAP.

Purity:

Purified

Form

Tissue culture supernatant containing 0.05% sodium azide.

Specificity:

Recognizes human TIRAP (TIR Domain-containing Adaptor Protein)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 4 Horng, T., Barton, G., Medzhitov, R. TIRAP: and adapter molecule in the Toll signaling pathway. Nature . Sept 2001, vol. 2, No. 9 835-841.