Technical Data
T5500-01M
TIRAP, CT (Toll-interleukin 1 Receptor Domain Containing Adapter Protein, TIR Domain-containing Adapter Protein, Adaptor Protein Wyatt, MyD88 Adapter-like Protein, Mal, Tlr4ap)
Description:
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-?B and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human heart cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to murine TIRAP (CT).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes mouse TIRAP/Mal (CT). Species Crossreactivity: rat and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. 5. McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82. 6. Yamamoto Y, Sato S, Hemmi H, et al. Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4. Nature 2002; 420:324-9. 7. Hoorng T, Barton GM, FlavellRA, et al. The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors. Nature 2002; 420:329-333.