Technical Data
TIRAP (MAL, Toll-interleukin 1 Receptor Domain containing Adaptor Protein, TIRAP)
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.

Suitable for use in Western Blot. and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml
Immunohistochemistry: 2ug/ml
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
Sheep anti rabbit IgG: HRP

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to a region within the C-Terminus of mouse TIRAP.
Purified by Immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Recognizes mouse TIRAP (toll-interleukin 1 receptor domain containing adaptor protein), also known as Mal. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. ONeill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.