Technical Data
T5595-0771
Tissue cDNA Panel, Human Adult Normal, Reproductive 3, Prostate, Testis, Ovary, Uterus, Placenta, BioGenomics™
5Panels
Molecular Biology Storage: -20°CShipping: Dry Ice
BioGenomics™ Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.

The cDNA panel is comprised of 5 tubes each containing 10ul of PCR Ready First Strand cDNAs from 5 tissues. These cDNAs are ideal for analyzing gene expression patterns and discovering novel genes. The cDNAs included in the panel are subject to source availability. All cDNAs in a panel are made under optimal and consistent conditions, which ensure that representation is maintained from total RNA to cDNA.

The First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetel normal tissues, human diseased and tumor tissues, as well as from mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is in 1X RT buffer (1X RT Buffer: 50 mM Tris Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT).

1ul cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs.

Features:
Ready to use for PCR quantification of gene expression
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs
The largest selection of cDNAs from different tissues on the market
Placenta cDNA is included in every panel as an interpanel control
Documentation of tissues' clinical histories available (additional cost)
Economical way to get cDNA from multiple tissues

Applications:
Immediate PCR amplification of known genes for their expression
Quantification of low copy gene expression in multiple tissues
Identification of tissue-specific expression of target genes
Analysis of gene expression in rare tissues
Verification of genetic mutation
Comparison of a specific gene between different tissues
Analysis of mRNA alternative splicing

Panel Components:
T5595-0771A: Prostate Normal 1x10rxn
T5595-0771B: Testis Normal 1x10rxn
T5595-0771C: Ovary Normal 1x10rxn
T5595-0771D: Uterus Normal 1x10rxn
T5595-0771E: Placenta Normal 1x20rxn
T5595-0010: Actin control primer 1x25uM

Storage and Stability:
Store cDNA panel at -20ºC Stable for 12 months.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.