Technical Data
Media
TNM-FH Insect Medium w/L-Glutamine (Powder)
T7000
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/L
Inorganic Salts:
Calcium Chloride1.000
Magnesium Chloride1.068
Magnesium Sulfate, anhy1.358
Potassium Chloride2.240
Sodium Phosphate Monobasic0.877
Amino Acids:
b-Alanine0.200
L-Alanine0.225
L-Arginine•HCl0.700
L-Asparagine0.350
L-Aspartic Acid0.350
L-Cystine•2HCl0.025
L-Glutamic Acid0.600
L-Glutamine0.600
Glycine0.650
L-Histidine2.500
L-Isoleucine0.050
L-Leucine0.075
L-Lysine•HCl0.625
L-Methionine0.050
L-Phenylalanine0.150
L-Proline0.350
DL-Serine1.100
L-Threonine0.175
L-Tryptophan0.100
L-Tyrosine•2Na0.072
L-Valine0.100
Vitamins:
p-Aminobenzoic Acid0.000020
D-Biotin0.000010
Choline Chloride0.000200
Folic Acid0.000020
Myo-Inositol0.000020
Niacin0.000020
D-Pantothenic Acid•Ca0.000020
Pyridoxine•HCl0.000020
Riboflavin0.000020
Thiamine0.000020
Other:
D(-)Fructose0.400
Fumaric Acid0.055
D(+)-Glucose0.700
a-Ketoglutaric Acid0.370
Lactalbumin Hydrolysate3.333
L(-)-Malic Acid0.670
Succinic Acid0.060
Sucrose26.680
Yeast Extract3.333
Total:51.192
A number of modifications of Grace's medium were developed in several laboratories in the late 1960's and 1970's. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper,Tricoplusia ni. This formula represents the modification known as TNM-FH. When properly supplemented, TNM-FH Insect Medium supports a number of cell lines from a variety of lepidopteran species. It is ideally suited for growth, infection, and protein expression of insect cell lines. This medium may be used for suspension or monolayer cultures. This medium is generally supplemented with serum.


Directions per Liter: Dissolve 51.19 grams in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 0.35 g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Beige to pale yellow, homogeneous, free flowing.

Solubility: Light yellow, clear, complete

pH: As reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Hink, W.F.: Established insect cell line from the cabbage, looperTricoplusia ni.. Nature 226: 466-467 (1970). 2. Hink, W.F., et al.: Effects of media constituents on growth of insect cells in stationary and suspension cultures. In Vitro 9: 371 (1974).