Technical Data
T7948-20
TOB2 (Protein Tob2, Transducer of erbB-2 2, Protein Tob4, KIAA1663, TOB4, TOBL, TROB2)
Description:
TOB2 is a 344aa rich novel member of the Tob:BTG1 anti-proliferative family of proteins characterized by similarities in their N-terminal BTG:Tob homology domains. This anti-proliferative protein inhibits cell cycle progression from the G0:G1 to S phases and is involved in cell cycle regulation through interaction with Caf1, a component of the CCR4 transcription factor complex that associates with cyclin-dependent kinases. Recent reports suggest that TOB2 plays important roles in spermatogenesis, embryonic dorsoventral patterning, osteogenesis, T-cell activation, learning and memory and acts primarily as a transcriptional repressor in several signaling pathways. TOB2, a signaling protein, may participate in transcriptional regulation of several genes. It is a regulatory protein ubiquitously expressed in most of the tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human heart lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa50-100 of human TOB2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human TOB2. Species Crossreactivity: chicken, chimpanzee, bovine, canine, equine, mouse, orangutan. Species sequence homology: xenopus, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ajima, R. et al. Gene. 253:215-220 (2000). 2. Ikematsu, N. et al. Oncogene. 18:7432-7441, 1999. 3. Jia, S. et al. Dev Dyn. 236:913-921 (2007).4. Kawamura-Tsuzuku, J. et al. Oncogene. 23:6630-6638 (2004).