Technical Data
Media
Todd Hewitt Broth (Powder)
T8030
Microbiological Grade
Storage RT/4C    Shipping RT
Components as grams per liter:
Beef Heart Infusion3.1
Yeast Enriched Peptone20.0
Dextrose2.0
Sodium Chloride2.0
Disodium Phosphate0.4
Sodium Carbonate2.5
Total:30g/liter
Broth medium intended for the cultivation and serological typing of group A hemolytic streptococci.

Directions per Liter:
Dissolve 30g in 800-900ml of dH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Direction for use: Mix 30 grams of the medium in one liter of dH2O until evenly dispersed. Heat with repeated stirring to dissolve completely Distribute and autoclave at 121.0C for 15 minutes.

Appearance: Light beige to beige, homogeneous, free flowing powder.

Solubility: Gold to amber, clear, light to no precipitate

pH: 7.80.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.