		Technical Data
T8050-01P	TLR1 (Toll Like Receptor 1, CD281, CD281 Antigen, KIAA0012, Toll/interleukin 1 Receptor-like Protein, TIL)

Description:
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR1 is co-expressed with TLR2 on myeloid cells of the innate immune systems in lymphoid tissue such as monocytes and dendritic cells where they form heterodimers that can recognize triacylated lipoproteins.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse spleen lysate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to amino acid sequences between 150-200 of human TLR1/Toll-like receptor 1 protein. (Q15399).

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human TLR1 (NT). Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003;3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003;21:335-76. 3. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002;20:197-216. 4. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003;24:286-9. 5. McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004;41:577-82. 6. Ochoa M-T, Legaspi AJ, Hatziris Z, et al. Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoid tissue. Immunol. 2003;108:10-15. 7. Takeuchi O, Sato S, Horiuchi T, et al. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J. Immunol. 2002;169:10-4.