Technical Data
T8050-09B
TLR2 (Toll Like Receptor 2)
Description:
The Toll-like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by researcher.

Hybridoma:
NSO myeloma cells with spleen cells from Balb/c mice.

IHC Positive Control Tissue:
Tonsil

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo

Recommended Negative Controls:
I1904-83P1: IgG2a Murine Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H3050Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
CHO cell line transfected with human TLR2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human toll-like receptor 2 (TLR2).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lien, E., et al., J. Biol. Chem. 274: 33419-25 (1999). 2.FloT. et al., J. Leukoc. Biol. 69: 474-481 (2001). 3.Flo T.et al., J. Immunol 164, 2064-2069 (2000).