Technical Data
T8050-17D
Toll Like Receptor 2 (TLR2, CD282)
Description:
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Immunohistochemistry (Frozen): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6A635Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.1mg/ml
Immunogen:
Mouse Toll Like Receptor 2 peptide
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes mouse Toll-like receptor 2 (TLR2, CD282). Stains overexpressed, as well as endogenous cell surface and intracellular TLR2. Does not affect cell activation through TLR2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meng, G et al; Murine TLR2 expression analysis and systemic antagonism by usage of specific monoclonal antibodies. Immunol Lett 2005, 98: 200.