		Technical Data
T8050-17F	Toll Like Receptor 2, NT (TLR2, CD282)

Description:
Toll Like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is also expressed in the spleen, bone marrow and lymph nodes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml; Detected a band of ~100kD in A-20 cell lysates.
Immunohistochemistry (Paraffin): 2ug; Does not require protein digestion pre-treatment of paraffin sections or antigen retrieval using heat treatment prior to staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Highly Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Peptide corresponding to an 14 amino acid sequence near the amino terminus of human TLR2.

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human Toll Like Receptor 2, NT. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Vogel, S.N., et al (2003) Mol. Interv. 3:466-77. 2. Takeda, K., et al (2003) Annu. Rev. Immunol. 21:335-76. 3. Janeway, C.A., et al (2002) Annu. Rev. Immunol. 20:197-216. 4. O’Neill, L.A.J, et al (2003) Trends in Imm. 24:286-9.