Technical Data
T8050-17F
Toll Like Receptor 2, NT (TLR2, CD282)
Description:
Toll Like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is also expressed in the spleen, bone marrow and lymph nodes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml; Detected a band of ~100kD in A-20 cell lysates.
Immunohistochemistry (Paraffin): 2ug; Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding to an 14 amino acid sequence near the amino terminus of human TLR2.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human Toll Like Receptor 2, NT. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vogel, S.N., et al (2003) Mol. Interv. 3:466-77. 2. Takeda, K., et al (2003) Annu. Rev. Immunol. 21:335-76. 3. Janeway, C.A., et al (2002) Annu. Rev. Immunol. 20:197-216. 4. ONeill, L.A.J, et al (2003) Trends in Imm. 24:286-9.