Technical Data
TLR3 (Toll Like Receptor 3)
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). mTLR3 cDNA codes for a protein with approximate molecular weight of 100kD . TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-5). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml, Mouse spleen whole cell lysate can be used as a positive control.
Immunohistochemistry (paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceMouseRabbit
A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes mouse TLR3. Predicted to react with rat TLR3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med . 187: 2097-2101 (1998). 2.Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3.Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 4. Rock FL., Hardiman G., Timans JC., Kastelein RA. and Bazan JF. Proc. Natl. Acad. Sci . U.S.A. 95 (2), 588-593 (1998). 5.Muzio M, et al. J Leukoc Biol 67(4):450-456 (2000). 6.Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A. J Immunol 164(11):5998-6004 (2000).