Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria.
Suitable for use in Flow Cytometry, Functional Studies, ELISA, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|50ug||4°C (-20°C Glycerol)||Blue Ice||Human||Mouse|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% chloroacetamide. Labeled with Biotin.|
|Recognizes human TLR4 (CD284) that is associated with MD-2. This clone is a TLR4 (CD284) function-blocking antibody that is useful for studies on the role of TLR4 (CD284) as a receptor for LPS induced cytokine production by TLR4 bearing cells. Species crossreactivity: canine, cynomolgus monkey, rhesus monkey and marmoset monkey.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Shimazu, R et al; MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med (1999) 189: 1777. 2. Tabeta, K et al; Toll-like receptors confer responsiveness to lipopolysaccharide from porphyromonas gingivalis in human gingival fibroblasts. Infect Immun (2000) 68: 3731. 3. Akashi, S et al; Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun (2000) 268: 172. 4. Wang, JE et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun (2001) 69: 2402. 5. Triantafilou, M et al; Lateral diffusion of toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane. J Cell Sci (2004) 117: 4007.|