Technical Data
T8050-42
TLR5 (Toll Like Receptor 5, Toll-like Receptor 5 Precursor, TLR 5, TLR-5, FLJ10052, MGC126430, MGC126431, Toll/interleukin 1 Receptor-like Protein 3, TIL3)
Description:
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3).

Applications:
Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug per 1x10e6 cells
Western Blot: 1-3ug/ml; 60kD band is detected in transfected cells, corresponding to a truncated form of TLR5.
Immunohistochemistry (Frozen): 10ug/ml
Immunohistochemistry (Paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k4H281Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human TLR5. Species Crossreactivity: mouse and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muzio, M., et al., Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov, R. and Janeway, C.A., Cell 91: 295-298 (1997). 3. Chuang, T.H. and Ulevitch, R.J., Biochim. Biophys. Acta 1518(1-2): 157-161 (2001).