Technical Data
T8050-84C
TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600) (FITC)
Description:
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kD.

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry (Intracellular): 0.5-2ug per 10e6 cells
Flow Cytometry (Cell surface): see Xu et al. (2006)
Western Blot: 1-3ug/ml unconjugated antibody
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG16D83Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS pH7.2, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human TLR8 at ~120kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Xu, S., et al., (2006) Surgery, 140:170.