Technical Data
TLR9 (Toll Like Receptor 9, CD289) (PE)
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TRL9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen.

Suitable for use in Flow Cytometry and Immunohistochemistry. Reported by an independent laboratory to be suitable for Confocal Microscopy. Other applications not tested.

Recommended Dilutions:
Flow Cytometry (Intracellular): 0.1-0.5ug/1x10e6 cells
Immunohystochemistry (Frozen): 10-20ug/ml
Immunohistochemistry (Paraffin): Reported by an independent laboratory to be suitable for use on paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
Ramos, human PBMCs

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
MabIgG1,k4H286Affinity Purified
100ug4C Do Not FreezeBlue IceHumanMouse
Synthetic peptide corresponding to aa268-284, CPRHFPQLHPDTFSHLS of human TRL9, conjugated to KLH.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Recognizes human TLR9 (Toll Like Receptor 9). Species Crossreactivity: mouse, rat, canine, equine and Rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muzio, M., et al., J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov, R. & Janeway, C.A., Cell 91: 295-298 (1997). 3. Chuang, T.H. & Ulevitch, R.J., Biochim. Biophys. Acta 1518(1-2): 157-161 (2001). 4. Takeuchi, O., et al., Gene 231: 59-65 (1999). 5. Hemmi, H., et al., Nature 408: 740-745 (2000). 6. Lee, H-K., et al., J. Biol. Chem. 279(11): 10,564-10,574 (2004).