Technical Data
T8050-94
TLR9 (Toll Like Receptor 9, CD289) (PE)
Description:
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TRL9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Reported by an independent laboratory to be suitable for Confocal Microscopy. Other applications not tested.

Recommended Dilutions:
Flow Cytometry (Intracellular): 0.1-0.5ug/1x10e6 cells
Immunohystochemistry (Frozen): 10-20ug/ml
Immunohistochemistry (Paraffin): Reported by an independent laboratory to be suitable for use on paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
Ramos, human PBMCs

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG1,k4H286Affinity Purified
SizeStorageShippingSourceHost
100ug4°C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa268-284, CPRHFPQLHPDTFSHLS of human TRL9, conjugated to KLH.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human TLR9 (Toll Like Receptor 9). Species Crossreactivity: mouse, rat, canine, equine and Rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muzio, M., et al., J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov, R. & Janeway, C.A., Cell 91: 295-298 (1997). 3. Chuang, T.H. & Ulevitch, R.J., Biochim. Biophys. Acta 1518(1-2): 157-161 (2001). 4. Takeuchi, O., et al., Gene 231: 59-65 (1999). 5. Hemmi, H., et al., Nature 408: 740-745 (2000). 6. Lee, H-K., et al., J. Biol. Chem. 279(11): 10,564-10,574 (2004).