Technical Data
T8064-99
Topoisomerase I (DNA Topoisomerase 1, DNA Topoisomerase I, NUP98 Fusion Gene, TOP 1, TOP1, TOP-1, TOP I, TOPI, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase)
Description:
Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5ug/ml using HRP/ECL for detection.
Recommended blocking buffer: Casein/Tween 20-based blocking and blot incubation buffer

Positive Control: Cell lysate from untreated HepG2 cells.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.



TypeIsotypeCloneGrade
MabIgG16D803
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa 699-725 of human topoisomerase 1.
Purity:
Purified from serum-free culture superantant by thiophilic adsorption and size exclusion chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, PEG and sucrose.
Specificity:
Recognizes human topoisomerase I.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.