Technical Data
T8064-99A
Topoisomerase I (DNA Topoisomerase 1, DNA Topoisomerase I, NUP98 Fusion Gene, TOP 1, TOP1, TOP-1, TOP I, TOPI, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: Extract of A231 human kidney cells.

Cellular Localization: Cytoplasmic and Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19F293Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~2.5mg/ml
Immunogen:
Full length protein (Human).
Purity:
Purified by Protein A affinity chromatography.
Form
15mM Sodium Azide; 0.01M PBS. pH 7.4
Specificity:
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Champoux JJ DNA topoisomerases: structure, function, and mechanism. Annu Rev Biochem 70:369-413 (2001). Takahashi H et al. Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis. Hybridoma 19:331-4 (2000). Froelich-Ammon SJ & Osheroff N Topoisomerase poisons: harnessing the dark side of enzyme mechanism. J Biol Chem 270:21429-32 (1995).