		Technical Data
T8064-99D	Topoisomerase I (DNA Topoisomerase 1, DNA Topoisomerase I, NUP98 Fusion Gene, TOP 1, TOP1, TOP-1, TOP I, TOPI, TOP-I, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, Topoisomerase-1, Topoisomerase-I, Type I DNA Topoisomerase) (Azide Free)

Description:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq]

Applications:
Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1.5ug/ml
Western Blot: 1:500-1:1,000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11C887	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant protein corresponding to aa692-766 of human TOP1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human TOP1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.