		Technical Data
T8065-03	TOP3B (DNA Topoisomerase 3-beta-1, DNA Topoisomerase III beta-1, TOP3B1, Topoisomerase (DNA) III beta) (Azide Free)

Description:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. [provided by RefSeq]

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2b	11C888	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant protein corresponding to aa1-863 of human TOP3B.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human TOP3B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.