Technical Data
T8065-10
Topoisomerase II alpha DNA
Description:
Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2-& M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo IIa is also implicated in drug resistance of tumor cells.

Applications:
Suitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (use Protein A): 10ug/mg protein lysate
Western Blot: 5-10ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.

Positive Control:
Raji cells or exponentially growing any cultured human cells.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
0.5mg/ml
Immunogen:
Synthetic peptide, corresponding to aa 1513-1530 (Cys-RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIa (KLH). Cellular Localization: Nuclear. MW of Antigen: 170kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol to 40%. Also supplied without BSA and Sodium Azide. See T8065-10X.
Specificity:
Recognizes a 170kD protein, which is identified as topoisomerase IIa. It shows no crossreaction with Topoisomerase IIb or I. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wang JC. DNA topoisomerases. Ann Rev Biochem 54: 665-697, 1985.
2.Earnshaw WC, Halligan B, Cooke CA, Heck MMS, and Liu LF. Topoisomerse II is a structural component of mitotic chromosome scaffolds. J Cell Biol 100: 1706-1715, 1985.
3.Earnshaw WC and Heck MMS. Localization of topoisomerse II in mitotic chromosomes. J Cell Biol 100: 1716-1725, 1985.