Technical Data
Topoisomerase II (DNA) alpha (DNA Topoisomerase 2 alpha, TOP2, TOP2A, TP2A)
Topoisomerase II alpha is one of the isoforms of the mammalian Topoisomerase II which has been implicated in many aspects of DNA metabolism, including transcription, replication, recombination, and segregation. It is preferentially expressed in proliferating cells and has been found to increase during S phase, peak at G2-M, and be diminished during G1. The gene encoding the human protein has been mapped to chromosome 17q21-22. It is an essential nuclear enzyme for chromosome segregation during mitosis. Mutations in the protein associated with drug resistance show clustering in exons 12, 13, 19-21 and 34-35. The pathological role of the enzyme has been identified the ataxia-telangiectasia.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:15-1:35
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20°CBlue IceHumanMouse
Not determined.
Synthetic polypeptide corresponding to the aa854-1447 of human topoisomerase II. Cellular Localization: Nuclear.
Supplied as a liquid, 0.05% sodium azide.
Recognizes the alpha form of human DNA topoisomerase II.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bauman et al. Mod Pathol 10: 168, 1997. 2. D’Andrea et al. Appl Immunohistochem 2: 177, 1994. 3. Robinson et al. Hybridoma 12: 401, 1993. 4. Lang et al.Gene 221: 255, 1998. 5. Null et al. Cell Growth & Differentiation 13: 325, 2002. 6. Dage Liu et al. Cancer 94: 2239, 2002.