Technical Data
Topoisomerase II (DNA) beta (TOP IIB, Top2 beta, Antigen MLAA 44, U937-associated Antigen)
Topoisomerase II beta plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is unregulated in tumors and proliferating cells. Topoisomerase II beta is also implicated in drug resistance of tumor cells.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: Tonsil

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
500ul-20CBlue IceHumanRabbit
Not determined
Corresponding to aa 300 of the C-terminus of human Topoisomerase II beta protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM PBS, BSA. pH 7.4, 0.09% sodium azide.
Detects a band at approximately 300kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.