Technical Data
T8065-21F-FITC
TOP2A (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2, Topoisomerase (DNA) II alpha 170kD) (FITC)
Description:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq]

Applications:
Suitable for use in FLISA, Immunofluorescence, Immunohistochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
FLISA: 3ng/ml-1ug/ml
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k11C889Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Partial recombinant protein corresponding to a region within aa1435-1532 of human TOP2A (NP_001058), with a GST tag. MW of GST tag alone is 26kD.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.
Specificity:
Recognizes human TOP2A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.