Technical Data
Torc1 (Transducer of Regulated CREB Activity 1)
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic beta-cells through a glucose sensing pathway. Feeding also stimulates the release of gut hormones such as glucogon-like peptide-1 (GLP-1), which induces insulin release, inhibits glucagon release and promotes beta-cell viability. Studies suggest a role for CREB-dependent transcription in glucose sensing and GLP-1 signaling (1). Furthermore, Torc2 (transducer of regulated CREB activity 2), a co-activator of CREB (2,3), is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and is consequently sequestered in the cytoplasm via its interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its subsequent dissociation with 14-3-3 proteins. Dephosphorylated Torc2 then enters into the nucleus and promotes CREB-dependent transcription. Torc2 is also shown to play a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). In addition, two Torc2-related proteins, Torc1 and Torc3, have been identified (2,3). Both Torc1 and Torc3 are also CREB co-activators (3). Torc1, Torc2 and Torc3 have been shown to associate with Tax directly and promote Tax-dependent transcription of human T-cell leukemia virus type I long terminal repeats (6,7).

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western blot 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH-coupled) derived from the sequence of human Torc1 (around G296).
Purified by protein A affinityt chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Detects endogenous levels of total Torc1 protein. Species sequence homology: Human - 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Hinke, S.A. et al. (2004) J. Physiol. 558, 369380.(2) Conkright, M.D. et al. (2003) Mol. Cell 12, 413423.(3) Iourgenko, V. et al. (2003) Proc. Natl. Acad. Sci. USA100, 1214712152.(4) Screaton, R.A. et al. (2004) Cell 119, 6174.(5) Koo, S.H. et al. (2005) Nature 437, 11091111.(6) Koga, H. et al. (2004) J. Biol. Chem. 279, 5297852983.(7) Siu, Y.T. et al. (2006) J. Virol. 80, 70527059.