Technical Data
Torc1 (Transducer of Regulated CREB Activity 1)
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic -cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes -cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). Torc1 and Torc3, a pair of Torc2-related proteins, also act as CREB coactivators (2,3). Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Synthetic peptide (KLH-coupled) derived from the sequence around Val463 of human Torc1.
As reported
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/mlBSA, 50% glycerol, 0.02% sodium azide.
Detects endogenous levels of total Torc1 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Hinke, S.A. et al. (2004) J. Physiol. 558, 369380.
(2) Conkright, M.D. et al. (2003) Mol. Cell 12, 413423.
(3) Iourgenko, V. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 1214712152.
(4) Screaton, R.A. et al. (2004) Cell 119, 6174.
(5) Koo, S.H. et al. (2005) Nature 437, 11091111.
(6) Koga, H. et al. (2004) J. Biol. Chem. 279, 5297852983.
(7) Siu, Y.T. et al. (2006) J. Virol. 80, 70527059.