Technical Data
T8067-55B
Torc1 (Transducer of Regulated CREB Activity 1)
Description:
TORC1, also known as CREB-regulated transcription coactivator 1 (crtc1), is a 67kD member of the TORC family. Mouse TORC1 is 630aa in length. It contains multiple potential sites for phosphorylation and a serine-rich region from aa295-365. Mouse TORC1 is 97% and 90% aa identical to rat and human TORC1, respectively. TORC1 is highly expressed in specific regions of the brain including the cortex, hippocampus, and striatum. It functions as a transcription coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cells with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse TORC1 (aa384-471).

Storage and Stability:
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2A9L860Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRat
Concentration:
As reported
Immunogen:
E. coli-derived, recombinant mouse TORC1 (aa384-471; Accession # Q68ED7).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes mouse TORC1. Species sequence homology: rat (97%), human (90%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.