Technical Data
Toxoplasma gondii
Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates. It completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild. Ssevere infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 30 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections at 1mM EDTA, pH 8.0., for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Infected brain tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceRabbit
Not Determined
Intact Toxoplasma gondii. Cellular Localization: Whole organism
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See T8075X.
Recognizes Toxoplasma gondii. Species crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Meseck, E.K. et al., (2005) J Vet Diagn Invest 17:565-568. 1. Conley, F.K., et al., Human Pathology 12(8): 690-698 (1981, Aug).