Technical Data
T8075-01A
Toxoplasma gondii (HRP)
Description:
Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Applications:
Suitable for use in ELISA, Immunoblot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA:1:200-1:1000
immunocytochemistry:1:20-1:200
Immunoblot: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceRabbit
Concentration:
~1-2mg/ml
Immunogen:
RH strain of T. gondii.
Purity:
Purified.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP.
Specificity:
Recognizes purified trophozoites.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.