Technical Data
T8075-30
Toxoplasma gondii
Description:
Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a1.B.516Affinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouse
Concentration:
~2mg/ml
Immunogen:
Tachyzoites lysate
Purity:
Purified by Protein G affinity chromatography. Purity tested by electrophoresis.
Form
Supplied as a liquid in PBS, pH 7.4 with 0.1% sodium azide.
Specificity:
Reacts with p30 membrane protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.