Technical Data
T8171-91
TRAF1 (TNF Receptor-associated Factor 1, Epstein-Barr Virus-induced Protein 6, EBI6, MGC:10353)
Description:
The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1:TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. Recognizes TRAF1; human TRAF1 is a 416aa protein.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000
Immunoprecipitation: 1:50-1:200
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Control:
Spleen, lymphoid, many cancer cell lines

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa1-22 (MASSSGSSPRPAPDENEFPFG) of human TRAF1, GenBank no. |NP_005649.1. The peptide sequence is 90% conserved with cow and dog TRAF1.
Purity:
Serum
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human TRAF1. Species sequence homology: bovine, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chung JY, YC Park, H Ye and H Wu. 2002. All TRAFs are not created equal:common and distinct molecular mechanisms of TRAF-mediated signal transduction. J Cell Science 115:679-688.