Technical Data
TRAF2, NT (TNF Receptor-associated Factor 2, E3 Ubiquitin-protein Ligase TRAF2, MGC:45012, Tumor Necrosis Factor Type 2 Receptor-associated Protein 3, TRAP3, TRAP)
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress. TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved. For example, TRAF2 and TRAF3 directly bind to the CD40, a NF receptor superfamily member involved in inducing B cell immunity, and are critical for NF-kB activation in mouse B lymphocytes. TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells. TRAF2 also interacts with the TRFR superfamily member lymphotoxin-b receptor (LTbR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human liver tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to 16aa peptide from near the N-terminus of human TRAF2.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human TRAF2. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arch, RH. et al. Genes Dev. 12, 2821 (1998). 2. van Kooten, C. et al. J. Leukoc. Biol. 67, 2 (2000). 3. Grech, AP. et al. Immunity 21, 629 (2004). 4. Davies, CC. et al. Mol. Cell. Biol. 25, 9806 (2005). 5. Kuai, J. et al. J. Biol. Chem. 278, 14363 (2003).