Technical Data
TRAF2 (TNF Receptor-associated Factor 2, E3 Ubiquitin-protein Ligase TRAF2, AI325259)
The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1:TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. Recognizes TRAF2. TRAF2 is typically described as a 501aa protein, GenBank no. NP_033448.2. . However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed, please see the Application Notes for additional details. Detects TRAF2 isoforms containing the peptide immunogen sequence listed in the Antigen section.

Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000
Immunoprecipitation: 1:50-1:200
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas, spleen, lymphoid, many cancer cell lines

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceMouseRabbit
Not determined.
Synthetic peptide corresponding to aa250-271(CSFLEAQASPGTLNQVGPELLQR) of mouse TRAF2, GenBank no. NP_033448.2.
Supplied as a liquid, 0.05% sodium azide.
Recognizes mouse TRAF2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chung JY, YC Park, H Ye and H Wu. 2002. All TRAFs are not created equal:common and distinct molecular mechanisms of TRAF-mediated signal transduction. J Cell Science 115:679-688.