		Technical Data
T8171-96B	TRAF2 Binding Protein (TRAF2-binding Protein, T2BP, Putative MAPK-activating Protein PM14, Putative NF-kappa-B-activating Protein 20, TRAF-interacting Protein with Forkhead-associated Domain, TRAF-interacting Protein with FHA Domain-containing Protein A,

Description:
TIFA is a 184aa tumor necrosis factor receptor-associated factor 6 (TRAF)-interacting protein consisting of a forkhead-associated (FHA) domain that binds phosphothreonine, phosphoserine and a consensus TRAF6-binding motif. It activates NF-kB and c-JUN N-terminal kinase (JNK) and induces the oligomerization and polyubiquitination of TRAF6, which in turn activates TAK1 and IKK through a proteasome-independent mechanism. It acts as an adapter protein that links tumor necrosis factor receptor-associated factor 6 (TRAF6) to interleukin-1 (IL-1) receptor-associated kinase-1 (IRAK-1) in IL-1 receptor signaling. It is expressed widely in tumor cells.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined.

Immunogen:

Synthetic peptide corresponding to aa167-182 (SLCSSQSSSPTEMDEN) of human TIFA.

Purity:

Serum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

Specificity:

Recognizes human TIFA. Species sequence homology: chimpanzee, monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ea, CK. et al. Proc. Natl. Acad. Sci. USA . 101:15318-15323 (2004). 2. Matsuda, A. et al. Oncogene. 22:3307-3318 (2003).