Technical Data
T8171-96B
TRAF2 Binding Protein (TRAF2-binding Protein, T2BP, Putative MAPK-activating Protein PM14, Putative NF-kappa-B-activating Protein 20, TRAF-interacting Protein with Forkhead-associated Domain, TRAF-interacting Protein with FHA Domain-containing Protein A,
Description:
TIFA is a 184aa tumor necrosis factor receptor-associated factor 6 (TRAF)-interacting protein consisting of a forkhead-associated (FHA) domain that binds phosphothreonine, phosphoserine and a consensus TRAF6-binding motif. It activates NF-kB and c-JUN N-terminal kinase (JNK) and induces the oligomerization and polyubiquitination of TRAF6, which in turn activates TAK1 and IKK through a proteasome-independent mechanism. It acts as an adapter protein that links tumor necrosis factor receptor-associated factor 6 (TRAF6) to interleukin-1 (IL-1) receptor-associated kinase-1 (IRAK-1) in IL-1 receptor signaling. It is expressed widely in tumor cells.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa167-182 (SLCSSQSSSPTEMDEN) of human TIFA.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human TIFA. Species sequence homology: chimpanzee, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ea, CK. et al. Proc. Natl. Acad. Sci. USA . 101:15318-15323 (2004). 2. Matsuda, A. et al. Oncogene. 22:3307-3318 (2003).