Technical Data
T8172-21A
TRAF3 (TNF Receptor-associated Factor 3, CAP1, CAP-1, CD40 Receptor-associated Factor 1, CRAF1, CD40-binding Protein, CD40BP, LMP1-associated Protein 1, LAP1)
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Technical Data |
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T8172-21A |
TRAF3 (TNF Receptor-associated Factor 3, CAP1, CAP-1, CD40 Receptor-associated Factor 1, CRAF1, CD40-binding Protein, CD40BP, LMP1-associated Protein 1, LAP1) |
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Description: The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1:TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000 Immunoprecipitation: 1:50-1:200 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: Spleen, lymphoid, CA cell lines Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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