Technical Data
T8172-21C
TRAF3, CT (TNF Receptor-associated Factor 3, CAP1, CAP-1, CD40 Receptor-associated Factor 1, CRAF1, CD40-binding Protein, CD40BP, LMP1-associated Protein 1, LAP1)
Description:
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor:Toll-like receptor (IL-1:TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-?B. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-? receptor (LT?R) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LT?R.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to human TRAF3/TNF receptor-associated factor 3/CD40 binding protein/LMP1 associated protein 1/CD40BP/LAP1 (CT).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TRAF3 (CT). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) a family of adaptor proteins that regulate life and death. Genes Dev. 1998;12:2821-30. 2. Cheng G, Cleary AM, Ye Z, et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 1995;267:1494-8. 3. Mosialos G, Birkenbach M, Yalamanchili R, et al. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995;80:389-99. 4. van Eyndhoven WG, Gamper CJ, Cho E, et al. TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0kappaB activation. Mol. Immunol. 1999;36:647-58. 5. Kuai J, Nickbarg E, Wooters J, et al. Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin- receptor reveals a novel mechanism of apoptosis. J. Biol. Chem. 2003;278:14363-9.