Technical Data
T8172-21E
TRAF3 (TNF Receptor-associated Factor 3, CAP-1, CD40 Receptor-associated Factor 1, CRAF1, CD40-binding Protein, CD40BP, LMP1-associated Protein 1, LAP1, CAP1, CRAF1)
Description:
TRAFs (TNF receptor associated proteins) form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to region of rat TRAF3.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes rat TRAF3 at ~67kD. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.